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| Brand | MultiSciences |
| CatNum | 70-EK380 |
| Product Name | Rat IFN-gamma ELISA Kit |
| Customs Name | Rat IFN-gamma ELISA Kit |
| Application | ELISA |
| Reactivity | Rat |
| Assay Type | Sandwich ELISA |
| Suitable Sample Type | serum, plasma, cell culture supernates |
| Format | 96-well strip plate |
| Storage | 4 (unopened) standard stored at -20, others stored at 4 (opened) |
| Shipping Condition | 4 |
| Sample Volume | 20 l |
| Sensitivity | 1.03 pg/ml |
| Standard Curve Range | 31.25 - 2000 pg/ml |
| Spike Recovery Range | 87 % - 112 % |
| Mean Spike Recovery | 0.99 |
| CV of Intra plate | 4.1 % - 6.0 % |
| CV of Inter plate | 5.4 % - 6.6 % |
| Release Date | 2016/8/20 |
| Components | 96-well polystyrene microplate coatedwithamonoclonalantibodyagainstIFN- RatIFN-Standard,lyophilized IFN-DetectAntibody StandardDiluent Streptavidin-HRP AssayBuffer(10) Substrate(TMB) StopSolution WashingBuffer(20) PlateCovers |
| Describtion | This assay employs the quantitative sandwich enzyme immunoassay technique for the quantitative detection of rat IFN-. The Rat IFN- ELISA is for research use only. Not for diagnostic or therapeutic procedures. Interferon gamma (IFN-) is a dimerized soluble cytokine that is the only member of the type II class of interferons. IFN-, or type II interferon, is a cytokine that is critical forinnate and adaptive immunity against viral, some bacterial and protozoal infections. IFN- is an important activator of macrophages and inducer of Class II major histocompatibility complex (MHC) molecule expression. Aberrant IFN- expression is associated with a number of autoinflammatory and autoimmunediseases. The importance of IFN- in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFN- is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 Th1 and CD8 cytotoxic T lymphocyte (CTL) effector T cells onceantigen- specific immunity develops. The role of IFN- as a disease marker has been demonstrated for a number of different pathological situations including infections, autoimmune diseases, transplant rejection, allergy and diabetes. |
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